| dc.description.abstract | Aims: Determination of antibacterial efficacy of aqueous, methanol, dichloromethane, and hexane
extracts from Jeffreycia zeylanica leaves against Staphylococcus aureus, Escherichia coli, and
Pseudomonas aeruginosa.
Methodology: Completely ballooned leaves of J. zeylanica were gathered, air-dried, and milled
into fine powder. Then macerated in all four selected solvents for about 1-2 weeks, the extracts
were obtained by vacuum evaporation under reduced pressure. Antibacterial activity was performed
against Staphylococcus aureus (ATCC25923), Escherichia coli (ATCC 25922), and Pseudomonas
aeruginosa (ATCC 27853) using the agar well diffusion method and disc diffusion method. The
positive control utilized was Gentamicin. The inhibitory zone's diameter (in mm) was measured and
noted. The entire experiment was done in triplicates.
Results: Findings from the study indicated that methanolic leaves extract (EC50 39.03mg/mL) had
the highest effectiveness and potency against S. aureus using agar well diffusion method and,
methanolic leaves extract (EC50 2.301mg/mL) had the highest effectiveness and potency against S.
aureus using agar disc diffusion method.
Conclusion: This study designates that, leaves of J. zeylanica have potential antibacterial activity
using aqueous, methanol, Dichloromethane(DCM), and hexane extracts. Among them methanolic
extract showed the highest activity indicating the highest inhibition zones and the most susceptible
organism is S. aureus. Additional study is required to understand the mechanism and active
ingredients behind the antibacterial activity of further plant sections. | en_US |
