| dc.description.abstract | Mangifera indica (MI), locally known as ‘Amba’ in Sinhalese is the richest species of
Mangifera found in Sri Lanka with its multiple varieties. This research determined
the antioxidant and anti-inflammatory effects by in vitro bioassays of ethanol
extracts of the leaf (L) and stem/bark (SB) of three native MI varieties;
Karthucolomban (K), Willard (W), and Vellaicolomban (V). Ethanol extractions were
performed on the collected L and SB samples where, Total Phenolic Content (TPC),
Total Flavonoid content (TFC), antioxidant assays such as DPPH, ORAC, and an antiinflammatory
assay known as human red blood cell membrane stabilization assay
(HRBC), and also thin layer choreography (TLC) were conducted. When the sample
extracts were compared to gallic acid (GA) the highest TPC was recorded for KSB
3.33 mg/gGAE and KL 3.34 mg/gGAE. When compared against quercetin (Q) by the
TFC assay, WL demonstrated the highest flavonoid content (1.15 mg/gQE). DPPH
assay was conducted to determine the antioxidant activity, and results were
compared against ascorbic acid (AA). WSB showed the highest percentage inhibition
(95%), while the second highest inhibition was noted for WL (91%), and while AC
only showed 77% inhibition. ORAC assay was also conducted for these two samples
where it was found 0.83 mg/g AAE for WSB and 0.64 mg/g AAE for WL. Moreover,
when compared to ibuprofen (97% inhibition), the highest inhibition was noted for
VSB (97%), and KL and VL showed similar inhibitions (96%) by the HRBC assay.
Interestingly, KL showed two prominent spots by TLC. As per the findings, it can be
concluded that the tested three varieties of MI, do contain antioxidant and antiinflammatory
properties at varied levels. Nevertheless, WL and WSB showed good
antioxidant activity while VSB demonstrated noteworthy anti-inflammatory
activity. Thus, more detailed scientific research is warranted to effectively evaluate
the medicinal effects of all parts of these varieties of the MI plant. | en_US |
