| dc.description.abstract | The development of anti-inflammatory and antibacterial drugs is mainly dependent
on the discovery of novel compounds from natural sources. This study aims to
assess the in vitro anti-inflammatory and antibacterial activity of methanol and
aqueous extracts of leaf, bark and combination (leaf and bark) extracts of the plant
Horsfieldia iryaghedhi (RUK). RUK is a plant with variety of therapeutic uses and is
mainly used to treat stomach ulcers and indigestion. Both extracts were tested for
in vitro anti-inflammatory activity by the heat-induced egg albumin denaturation
method using Diclofenac Sodium as the positive control. The methanol bark, leaf and
combination extracts of Horsfieldia iryaghedhi exhibited the highest percentage
inhibition in its highest concentration (1000μg/ml) as 97.0±5.67%, 79.1±1.64% and
81.4±3.30% respectively while the percentage inhibition of Diclofenac Sodium was
85.0±3.37%. The aqueous bark, leaf and combination extracts of RUK also exhibited
the highest percentage inhibition in its highest concentration (1000μg/ml) as
67.6±5.53%, 80.4±2.55% and 77.0±2.49% respectively. All extracts were tested for
antibacterial activity against gram-negative bacteria Escherichia coli
(ATCC®25922) and gram-positive bacteria Staphylococcus aureus (ATCC®25923)
by agar well diffusion method using Gentamicin as the positive control. None of the
extracts were found to possess antibacterial activity against E.coli, but methanol leaf
and combination extracts at the highest concentration (100μg/ml) were observed
antibacterial activity against S.aureus with the zone of inhibition 19.6mm and
13.25mm respectively. This study concludes that the H.iryaghedhi extracts have
significant anti-inflammatory activity. Furthermore, methanol bark extract
demonstrated potent anti-inflammatory activity compared to other extracts. | en_US |
