Evaluation of Anti-inflammatory and Antibacterial activities of the extracts of leaves, roots and combination of leaves and roots of plant Magnolia figo.

Abstract

Abstract:-Discovery of novel drugs from medicinal plants is getting popular owing to lesser side effects and to overcome antimicrobial resistance. The methanolic extracts of both leaves and roots of Magnolia figo plant by cold maceration were subjected to the evaluation of anti-inflammatory and antibacterial activity. In vitro antiinflammatory property was determined using heat-induced protein (egg albumin) denaturation test compared to diclofenac sodium (positive control). Concentration series of the extracts were analyzed to calculate the percentage inhibition (IC50) of heat-induced protein denaturation. Antibacterial activity of the methanolic extract was determined against Escherichia coli (ATCC® 25922TM) and Staphylococcus aureus (ATCC® 25923TM) using the cylinder plate method using gentamycin as the positive control. The size of inhibitory zone was compared with the positive control to determine the antibacterial activity. Lower IC 50 value (1.819 (µg/mL)) was shown in the combination extract of M. figo plant compared to the reference drug (4.337 (µg/mL)). It reflects the synergistic effect of the plant parts. The leaves and roots combination extract exhibited dose-dependent behavior of antiinflammatory activity and highest antibacterial activity against E. coli (zone diameter – 15 mm). However, none of the extracts exhibited antibacterial activity against S. aureus. Phytochemical investigations of extracts indicated the presence of alkaloids, flavonoids, phenols, tannins, saponins, terpenoids, glycosides and steroids. Significant results elicited by the combination of plant parts confirm that M. figo is a medicinal plant which can be used to develop novel anti-inflammatory agents.