Gastroprotective Activity Of Vishnukarnthi Chewable Granular Dosage Form Formulated Using Evolvulus Alsinoides And Its Accelerated Stability Studies

Abstract

Abstract: This study investigates the in vitro gastroprotective activity of Vishnukranthi chewable granules formulated using dry plant powder of Evolvulus alsinoides by evaluating neutralizing capacity in artificial gastric juice and the titration method using Fordtran’s model. Accelerated stability studies were conducted upto three months to access the stability of granules. Each parameter was accessed at day 1, 1 month and after three months.Particle size distribution, moisture content and pH were measured as physical stability parameters. Microbial stability accessed by total viable bacteria and total viable fungi counts. Stability of the gastroprotective activity was evaluated using neutralizing capacity in artificial gastric juice and the titration method using Fordtran’s model. Chewable granules has demonstrated a significant (p<0.01) neutralizing capacity on artificial gastric acid (mean pH 1.71±0.01) when compared with negative control. The titration conducted using Fordtran’s model consumed 0.1493±0.0036 of H+ (p<0.001). There was a significant difference between fine percentage, percentage of weight loss, total viable count of granules at day 1 and after 1 month and 3 months (p<0.05). There were no significant differences between pH values, Rf values and the gastroprotective activity of granules at day 1 and after 1 month and 3 months. Granules were chemically stable but physically and microbiologically less stable. In conclusion, granules has demonstrated significant gastroprotective activity in both models. Further studies are recommended to improve the physical and microbial stability of the dosage form by adding a suitable binding agent and an appropriate preservative. Further, it is important to evaluate the efficacy of Vishnukranthi chewable granules clinically because this product has a good potential to commercialize as a herbal remedy for gastritis.