| dc.description.abstract | Metallothioneins (MT) are metal detoxifying
proteins that sequester metals. MT expression is
induced by metals such as As, Cd, and Zn. In a
cross sectional study, metallothionein expression
was followed in chronic kidney disease (CKD)
affected people in Padaviya area to investigate
whether the disease is etiologically linked to
metal exposure. Male subjects between 35-75
years of age volunteered for the study (n=202)
at CKD clinic, Base Hospital, Padaviya. Samples
were collected similarly at Padalangala for
a non-endemic control. Questionnaire based
information, whole blood and spot urine
samples were collected from each subject serum
Creatinine and Cystatin C and urine Albumin to
Creatinine ratio (UACR mg/g) were determined
by standard analytical procedures. eGFR (ml/
min-1.722m2) was calculated using MDRD and
EPI equations. Protein expression levels of MT-
1A and MT-2A genes were measured by enzyme
linked immunosorbent assay using polyclonal
mouse anti-human MT1A and monoclonal
mouse anti-human MT2A antibodies respectively.
Results showed that MT1A protein level of CKD
stage II was statistically different (p<0.05, one
way ANOVA followed by Tukey HSD) from both
stages III (p<0.002) and IV (p<0.004) whereas
MT2A protein levels were similar (p>0.05) among
all stages and control. Simple linear correlation
(Pearson’s) analyses reveled that MT1A protein
levels positively associated (p<0.05) with disease
progression in terms of serum Cystatin C based
(EPI) eGFR (r, 0.163) and serum Creatinine and
Cystatin C combined equation based (EPI) eGFR
(r, 0.171). Intra-stage analyses showed that MT1A
protein level was positively correlated (p<0.05)
to MDRD eGFR in stage II (r, 0.391), Cystatin C
based EPI eGFR in stage III (r, 0.381) and UACR
of 0-30 mg/g group (r, 0.284). In conclusion, MT-
1A expression appears to be modulated with the
disease progression in CKD patients in Padaviya
area. The study continues. | en_US |
