Anti-Glycation and Glycation Reversing Potential of Salacia Reticulata L. (Kothala Himbutu) Root, Stem, Leaf and Twig Extracts

Abstract

Glycation is a series of complex reactions between reducing sugars and proteins. This reaction ultimately produces multitude of detrimental advanced glycation end products (AGEs). Formation and accumulation of AGEs have been implicated in the development and progression of several diabetic complications, neurological diseases and aging. Thus, glycation inhibitors and glycation reversing agents offer a potential strategy as therapeutics for diverse diseases. Salacia reticulata L. is a scientifically well documented traditional anti-diabetic plant. However, anti-glycation and glycation reversing potential of this plant has not been studied. Present study reports anti-glycation and glycation reversing potential of Salacia reticulata. Freeze dried hot water extracts of Salacia reticulata root, stem, leaf and twigs were used in this study. Different concentrations of root, stem, leaf and twig extracts were subjected to anti-glycation and glycation reversing assays in vitro. Rutin was used as the positive control. Root, stem, leaf and twig extracts of Salacia reticulata showed significant (P < 0.05) anti-glycation activity in a dose dependent manner. IC50 values for anti-glycation activity of root, stem, leaf and twigs extracts were 13.06 ± 0.69, 27.29 ± 0.93, 144.53 ± 1.12 and 171.90 ± 0.88 ?g/ml respectively. Root extract showed significantly high (P < 0.05) anti-glycation activity compared to other extracts and rutin (IC50: 21.88 ± 2.82 ?g/ml). Glycation reversing potential of different parts of Salacia reticulata also showed significant (P < 0.05) and dose dependent relationship. EC50 values of root, stem, twig and leaf extracts were 101.60 ± 11.57, 116.67 ± 0.64, 180.53 ± 7.41 and 264.40 ± 9.30 ?g/ml respectively. Potency of different parts of Salacia reticulata for anti-glycation and glycation reversing activities were root > stem > leaf > twig and root = stem > twig > leaf respectively. It is concluded that all parts of Salacia reticulata possess both anti-glycation and glycation reversing activities. Further, this is the first study to report anti-glycation and glycation reversing potential of this plant.